rabbit anti il 5 polyclonal antibody  (Boster Bio)

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Carbamylated Erythropoietin Outperforms Erythropoietin in the Treatment of AKI-on-CKD and Other AKI Models

doi: 10.1681/ASN.2015091059

Figure Lengend Snippet: CD131 is expressed in the kidney. (A) In adult female Fisher rats, quantitative RT-PCR analysis of renal tissue at several time points after AKI showed that Epo mRNA levels were detectable in baseline (B.L.) controls (dashed line), dropped below detectable levels after AKI, and returned to baseline levels at 3 and 7 days post-AKI. (B) Epor mRNA levels fell below baseline levels at 2 and 24 hours and 3 days after AKI, and then, they returned toward baseline levels on day 7. (C) CD131 mRNA levels decreased and remained below baseline levels at 24 hours and 3 days, with a small but insignificant increase 7 days after AKI. *P<0.05 compared with the baseline control. (D) A Western blot for CD131 showed high expression in kidney and spleen tissues but not in the presence of a CD131–specific blocking peptide. CD131 has a molecular mass of approximately 130 kD (black arrow). Immunohistochemistry of kidney tissue showing (E) a negative control (Neg. Control) and (F) staining with CD131. (G) A magnified view of the area in the black box from E shows the negative control. (H) A magnified view of the area in the black box from F shows intensive CD131 staining of proximal tubular cells. n.d., not detectable. Scale bars, 100 μm in E and F; 200 μm in G and H.

Article Snippet: After incubation with a peroxidase-blocking reagent, slides were labeled with a 1:100 dilution of a primary anti–IL-3/IL-5/granulocyte macrophage colony-stimulating factor receptor β rabbit polyclonal antibody (K17) for CD131 (Santa Cruz Biotechnology) for 60 minutes and stained with the EnVision System (DAKO, Carpentaria, CA).

Techniques: Quantitative RT-PCR, Control, Western Blot, Expressing, Blocking Assay, Immunohistochemistry, Negative Control, Staining

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Carbamylated Erythropoietin Outperforms Erythropoietin in the Treatment of AKI-on-CKD and Other AKI Models

doi: 10.1681/ASN.2015091059

Figure Lengend Snippet: CD131 is expressed in the kidney. (A) In adult female Fisher rats, quantitative RT-PCR analysis of renal tissue at several time points after AKI showed that Epo mRNA levels were detectable in baseline (B.L.) controls (dashed line), dropped below detectable levels after AKI, and returned to baseline levels at 3 and 7 days post-AKI. (B) Epor mRNA levels fell below baseline levels at 2 and 24 hours and 3 days after AKI, and then, they returned toward baseline levels on day 7. (C) CD131 mRNA levels decreased and remained below baseline levels at 24 hours and 3 days, with a small but insignificant increase 7 days after AKI. *P<0.05 compared with the baseline control. (D) A Western blot for CD131 showed high expression in kidney and spleen tissues but not in the presence of a CD131–specific blocking peptide. CD131 has a molecular mass of approximately 130 kD (black arrow). Immunohistochemistry of kidney tissue showing (E) a negative control (Neg. Control) and (F) staining with CD131. (G) A magnified view of the area in the black box from E shows the negative control. (H) A magnified view of the area in the black box from F shows intensive CD131 staining of proximal tubular cells. n.d., not detectable. Scale bars, 100 μm in E and F; 200 μm in G and H.

Article Snippet: After incubation with a peroxidase-blocking reagent, slides were labeled with a 1:100 dilution of a primary anti–IL-3/IL-5/granulocyte macrophage colony-stimulating factor receptor β rabbit polyclonal antibody (K17) for CD131 (Santa Cruz Biotechnology) for 60 minutes and stained with the EnVision System (DAKO, Carpentaria, CA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Blocking Assay, Immunohistochemistry, Negative Control, Staining